Journal: Kidney International Reports
Article Title: Pauci-Immune Endocapillary Proliferative Glomerulonephritis With Glomerular M2 Macrophage Infiltration
doi: 10.1016/j.ekir.2026.103791
Figure Lengend Snippet: Multiplex immunofluorescence staining results (Case 3). (a) Macrophage subtypes and their distribution patterns. (b) CD86+ macrophages are sparsely distributed within the renal interstitium. (c–e) CD163+ and CD206+ macrophages are abundantly present in the interstitium, whereas CD163+ macrophages show less infiltration within the glomeruli. The scale bar in the figure represents 20 μm. DAPI, 4',6-diamidino-2-phenylindole.
Article Snippet: Formalin-fixed paraffin-embedded renal tissue sections (3.5 μm) were subjected to multiplex IF staining using the following primary antibodies: CD68 (ab955, pan-macrophage marker, Abcam), CD163 (ab182422, M2 macrophage marker, Abcam), CD86 (91882S, M1 macrophage marker, Cell Signaling Technology, Danvers, MA), CD206 (24595S, M2 macrophage marker, Cell Signaling Technology), CD56 (99746S, Cell Signaling Technology), CD3 (ab11089, Abcam), and CD8 (ab199016, Abcam).
Techniques: Multiplex Assay, Immunofluorescence, Staining